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Oshrat Levy-Ontman Merav Fisher Yoram Shotland Yacob Weinstein Yoram Tekoah Shoshana Malis Arad 《International journal of molecular sciences》2014,15(2):2305-2326
N-glycosylation is one of the most important post-translational modifications that influence protein polymorphism, including protein structures and their functions. Although this important biological process has been extensively studied in mammals, only limited knowledge exists regarding glycosylation in algae. The current research is focused on the red microalga Porphyridium sp., which is a potentially valuable source for various applications, such as skin therapy, food, and pharmaceuticals. The enzymes involved in the biosynthesis and processing of N-glycans remain undefined in this species, and the mechanism(s) of their genetic regulation is completely unknown. In this study, we describe our pioneering attempt to understand the endoplasmic reticulum N-Glycosylation pathway in Porphyridium sp., using a bioinformatic approach. Homology searches, based on sequence similarities with genes encoding proteins involved in the ER N-glycosylation pathway (including their conserved parts) were conducted using the TBLASTN function on the algae DNA scaffold contigs database. This approach led to the identification of 24 encoded-genes implicated with the ER N-glycosylation pathway in Porphyridium sp. Homologs were found for almost all known N-glycosylation protein sequences in the ER pathway of Porphyridium sp.; thus, suggesting that the ER-pathway is conserved; as it is in other organisms (animals, plants, yeasts, etc.). 相似文献
14.
B. Mishori E. A. Katz D. Faiman A. Belu-Marian Yoram Shapira 《Fullerenes, Nanotubes and Carbon Nanostructures》2013,21(1):113-124
Abstract The electronic structure of polycrystalline C60 thin films has been investigated using surface photovoltage spectroscopy (SPS) and conductivity measurements. the films show n-type semiconductivity with an activation energy of ~ 0.8 eV as found from the temperature dependence of the conductivity at high temperatures. the electronic structure emerging from our SPS results comprises a 1.6 eV photo-conduction gap, a mobility gap of about 2.25 eV and two gap states, a donor and an acceptor, at 0.35 eV and (1.0-1.1) eV, respectively, below the photo-conduction edge. the results indicate the possibility of the existence of band tails, extending into the optical gap of these films, as well as other deep gap states. 相似文献
15.
Astrid Heckl Samir Cenanovic Mathias G?ken Robert F. Singer 《Metallurgical and Materials Transactions A》2012,43(1):10-19
The phase stability of nickel-base superalloys has been investigated using a new in-house-designed alloy series with stepwise
increased additions of Re and Ru at otherwise fixed atomic fractions of alloying elements. Results presented in this study
are focused on the lesser-known topologically closed packed (TCP) formation of columnar colonies or so-called discontinuous
precipitation. A detailed investigation of these colonies allowed for identifying compositional changes during the growth
process and for providing a three-dimensional (3-D) illustration of the TCP phases within these colonies. The results were
used to compare the colony growth process with existing growth models. Furthermore, the influence of Re and Ru on the appearance
of discontinuous precipitation has been investigated by means of colony width and the effect on creep properties. Larson-Miller
plots are given to illustrate the creep strength of directionally solidified samples with and without TCP colonies compared
with single crystalline samples free from TCP colonies. 相似文献
16.
M. M. Franke R. M. Hilbinger C. H. Konrad U. Glatzel R. F. Singer 《Metallurgical and Materials Transactions A》2011,42(7):1847-1853
A numerical model was developed to estimate the solidification conditions and the secondary dendrite arm spacing of equiaxed
solidified IN738LC investment castings. The model, composed of geometric data, thermophysical properties, and boundary conditions,
was verified by a comparison of calculated and measured process temperatures obtained from casting experiments. The computation
of the secondary dendrite arm spacing was carried out from temperature gradient G, solidification rate v, and an alloy-specific parameter M, determined by means of an inverse approach. The calculated secondary dendrite arm spacing was found to be in very good agreement
with metallographic measurements. 相似文献
17.
Ralf Rettig Susanne Steuer Robert F. Singer 《Journal of Phase Equilibria and Diffusion》2011,32(3):198-205
Interdiffusion and impurity diffusion of Ge is studied in Ni and multicomponent alloys over the temperature range of 1150-1250 °C.
The diffusion is investigated using diffusion couples, which are evaluated using microprobe measurements. The interdiffusion
coefficients are calculated with the den Broeder method, while the impurity diffusion coefficients are determined with the
Hall method. A model for the diffusion simulation software DICTRA is developed by mobility assessments. Further on, the diffusion
of Ge in the multicomponent superalloys PWA1483 and René N5 is investigated. The Ge diffusion coefficient in René N5 is 4.5 × 10−14 m2/s at 1180 °C which is similar to other alloying elements such as Co, Cr, Mo or Ta. 相似文献
18.
We present a comprehensive robot development process and its evaluation. We designed this process in the context of a robotics course in high schools. The motivation for designing this new process was improving the robustness and reliability of robots developed by students and preparing students for becoming better designers. The newly designed process proved to be highly successful in designing top quality robots. In the process design, we explored and adapted existing design tools and methods to the specific designers, the nature of the product, the environment, the product needs, and the design context goals. At the end of this thorough design, we selected a synergetic integration of six tools and methods to compose the new comprehensive development process for this product context: conceptual design, fault-tolerant design, atomic requirements, fuzzy logic for control, creative thinking, and microprogramming-based design. The design skills of the students that learned the design process and the performance of robots they designed and participated in an international robotics contest were examined. The high school teams that studied the proposed process won the first places in an international contest. The robots developed by the students had better performance than robots built by engineers and faculty teams. Professional experts rated the robots’ designs as excellent. The students that studied the process demonstrated high level of diverse design skills including creativity and design management capabilities. Additionally, they improved their science subject grades and their attitude toward engineering. Both the results obtained by the study and the authors’ experience in teaching robotics demonstrate that the proposed robot development process could be taught successfully in high school and that it leads to superior robotic products. Our experience also indicates that this process could serve industry design by improving the robustness of robots operating in uncertain environments and supporting fast change management practices. 相似文献
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Ostroverkhov V. Ostroverkhova O. Petschek R.G. Singer K.D. Sukhomlinova L. Twieg R.J. 《IEEE journal of selected topics in quantum electronics》2001,7(5):781-792
This paper describes the development and optimization of chiral, nonpolar media with large second-order nonlinear optical responses. We employ molecular engineering, quantum-mechanical sum-over-states theory, and measurements of molecular hyperpolarizability by means of Kleinman-disallowed hyper-Rayleigh scattering in order to understand molecular properties. Then we analyze the appropriate arrangement of the chromophores that produce an optimum axial nonlinear optical medium. Chromophores with large Kleinman disallowed traceless symmetric second-rank tensor hyperpolarizabilities β can be aligned so as to result in large susceptibilities χ(2) in structures that lack polar order. We found that Λ-shaped chromophores with C2v or similar symmetry are good candidates for these materials, as they can exhibit large second-rank components of the hyperpolarizability tensor. A wide variety of techniques can be used to fabricate bulk materials belonging to the chiral nonpolar symmetry groups D∞ and D2. The microscopic chromophore alignment schemes that optimize the nonlinear optical response in such materials are deduced from general symmetry consideration for both molecules and bulk. We also speculate on the possible application of such materials as high-bandwidth spatial light modulators 相似文献